Rat GLP1Glucagon Like Peptide 1 ELISA Kit

Rat GLP-1(Glucagon Like Peptide 1) Introduction
Glucagon-like peptide-1 (GLP-1) is a 30- or 31-amino-acid-long peptide hormone deriving from the tissue-specific posttranslational processing of the proglucagon peptide. It is produced and secreted by intestinal enteroendocrine L-cells and certain neurons within the nucleus of the solitary tract in the brainstem upon food consumption. The initial product GLP-1 (1 37) is susceptible to amidation and proteolytic cleavage, which gives rise to the two truncated and equipotent biologically active forms, GLP-1 (7 36) amide and GLP-1 (7 37). Active GLP-1 protein secondary structure includes two -helices from amino acid position 13 20 and 24 35 separated by a linker region. Alongside glucose-dependent insulinotropic peptide (GIP), GLP-1 is an incretin; thus, it has the ability to decrease blood sugar levels in a glucose-dependent manner by enhancing the secretion of insulin. Beside the insulinotropic effects, GLP-1 has been associated with numerous regulatory and protective effects. Unlike GIP, the action of GLP-1 is preserved in patients with type 2 diabetes and substantial pharmaceutical research has therefore been directed towards the development of GLP-1-based treatment.

Rat GLP-1(Glucagon Like Peptide 1) ELISA Kit Test Method
This kit uses sandwich ELISA to detect the concentration of Rat GLP-1.

Rat GLP-1-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Rat GLP-1 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming opticalsignal measured by microplate reader.The concentration of Rat GLP-1 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.